Recent breakthroughs in transgenic technology, especially those based on the CRISPR/Cas system, have greatly boosted research in plant biology, agriculture, and biotechnology. CRISPR-mediated gene editing, with its high precision and versatility, has great potential for accelerating basic plant science research and crop improvement through the creation of multiple types of genetic variants and functionally optimized gene alleles. It has great potential for accelerating basic plant science research and crop improvement. The first step in plant transformation is the delivery of exogenous DNA into recipient cells. This step in plant gene editing enables the expression and function of CRISPR/Cas reagents inside the cell, including single-guide RNA (sgRNA) and Cas DNA nuclease. This involves a lengthy, expensive, and labor-intensive tissue culture step and is currently only possible in a limited number of plant species, making it a significant bottleneck in plant gene editing.
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